Cell adhesion to extracellular matrices is critical to numerous cellular functions and is primarily mediated by integrin receptors. Binding and aggregation of integrins leads to the formation of focal adhesions (FA) which connect the cytoskeleton to the extracellular matrix in order to reinforce adhesion and transmit signals [1]. Preliminary observations indicated preferential recruitment of FAs to the periphery of the cell spreading area on both uniformly coated and micropatterned fibronectin surfaces (Fig. 1). The current study investigates the biophysical regulation of cell adhesion strength based on the size and position of FA with the central hypothesis that peripheral FAs stabilize adhesion strength. The hypothesis was tested by delineating the cell spreading area from the total cell adhesive area by employing microcontact printing to pattern substrates with a series of circular and annular adhesive islands which control cell shape (Fig. 2). A well characterized hydrodynamic shear assay known as the spinning disk device was used to quantify the adhesion strength of cells adhered to the micropatterns [2].
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Regulation of Adhesion Strength by Focal Adhesion Position and Cell Shape Available to Purchase
Nathan D. Gallant,
Nathan D. Gallant
University of South Florida, Tampa, FL
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Kranthi Kumar Elineni
Kranthi Kumar Elineni
University of South Florida, Tampa, FL
Search for other works by this author on:
Nathan D. Gallant
University of South Florida, Tampa, FL
Kranthi Kumar Elineni
University of South Florida, Tampa, FL
Paper No:
SBC2012-80832, pp. 693-694; 2 pages
Published Online:
July 19, 2013
Citation
Gallant, ND, & Elineni, KK. "Regulation of Adhesion Strength by Focal Adhesion Position and Cell Shape." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 693-694. ASME. https://doi.org/10.1115/SBC2012-80832
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