Intervertebral disc (IVD) degeneration is accompanied by elevated levels of pro-inflammatory cytokines, particularly IL-1β and TNF-α [1]. Disc cells from the nucleus pulposus (NPs) respond to cytokine stimulation with increased catabolic breakdown of the tissue, resulting in a positive feedback of disc integrity loss and further inflammation [2]. Previous studies by our group have examined the response of NP cells to Toll-Like Receptor-4 (TLR-4) activation through stimulation with lipopolysaccharide (LPS). TLR-4 is a pattern recognition receptor that is activated in innate immunity and by polysaccharide fragments from degenerated proteoglycans. TLR-4 activation by LPS results in stimulation of multiple cytokines by NP cells [3]. Moreover, we have shown that in vivo LPS injection results in catabolic changes in the IVD, including matrix breakdown, decrease in biomechanical properties and loss of disc height [4]. However, the specific cellular mechanisms for these catabolic changes remain to be elucidated.
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Effect of Inflammation on the Osmotic Response of Nucleus Pulposus Cells
Robert Maidhof,
Robert Maidhof
The Feinstein Institute for Medical Research, Manhasset, NY
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Neena Rajan,
Neena Rajan
The Feinstein Institute for Medical Research, Manhasset, NY
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Nadeen O. Chahine
Nadeen O. Chahine
The Feinstein Institute for Medical Research, Manhasset, NY
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Robert Maidhof
The Feinstein Institute for Medical Research, Manhasset, NY
Neena Rajan
The Feinstein Institute for Medical Research, Manhasset, NY
Nadeen O. Chahine
The Feinstein Institute for Medical Research, Manhasset, NY
Paper No:
SBC2012-80358, pp. 399-400; 2 pages
Published Online:
July 19, 2013
Citation
Maidhof, R, Rajan, N, & Chahine, NO. "Effect of Inflammation on the Osmotic Response of Nucleus Pulposus Cells." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 399-400. ASME. https://doi.org/10.1115/SBC2012-80358
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