Stem cells respond to many microenvironmental cues towards their decisions to spread, migrate, and differentiate and these cues can be incorporated into materials for regenerative medicine.1 In the last decade, matrix stiffness alone has been implicated in regulating cellular functions such as migration, proliferation and differentiation. With this in mind, a variety of natural and synthetic polymer systems were used in vitro to mimic the elasticity of native tissues. Despite helping to develop this important field and gather valuable information, these substrates are primarily static and lack the dynamic nature that is observed during many cellular processes such as development, fibrosis and cancer. Thus, it is of great interest to temporally manipulate matrix elasticity in vitro to better understand and develop strategies to control these biological processes. In this work, we utilize a sequential crosslinking approach (initial gelation via addition reaction, secondary crosslinking through light-mediated radical polymerization) to fabricate hydrogel substrates that stiffen (e.g., ∼3 to 30 kPa) either immediately or at later times and in the presence of cells. We demonstrate the utility of this technique by investigating the short-term (several minutes to hours) and long-term (several days to weeks) stem cell response to dynamic stiffening
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Dynamic Mechanical Properties Control Adult Stem Cell Fate Available to Purchase
Murat Guvendiren,
Murat Guvendiren
University of Pennsylvania, Philadelphia, PA
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Jason A. Burdick
Jason A. Burdick
University of Pennsylvania, Philadelphia, PA
Search for other works by this author on:
Murat Guvendiren
University of Pennsylvania, Philadelphia, PA
Jason A. Burdick
University of Pennsylvania, Philadelphia, PA
Paper No:
SBC2012-80062, pp. 315-316; 2 pages
Published Online:
July 19, 2013
Citation
Guvendiren, M, & Burdick, JA. "Dynamic Mechanical Properties Control Adult Stem Cell Fate." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 315-316. ASME. https://doi.org/10.1115/SBC2012-80062
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