Generation of human induced pluripotent stem cells (hiPSCs) from fibroblasts and other somatic cells represents a highly promising strategy to produce auto- and allo-genic cell sources for therapeutic approaches as well as novel models of human development and disease1. Reprogramming protocols involve transduction of the Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc into the parental somatic cells, followed by culturing the transduced cells on mouse embryonic fibroblast (MEF) or human fibroblast feeder layers, and subsequent mechanical dissociation of pluripotent cell-like colonies for propagation on feeder layers1, 2. The presence of residual parental and feeder-layer cells introduces experimental variability, pathogenic contamination, and promotes immunogenicity3. Similar to human embryonic stem cells (hESCs), reprogrammed hiPSCs suffer from the unavoidable problem of spontaneous differentiation due to sub-optimal feeder cultures4, growth factors5, and the feeder-free substrate6. Spontaneously differentiated (SD)-hiPSCs display reduced pluripotency and often contaminate hiPSC cultures, resulting in overgrowth of cultures and compromising the quality of residual pluripotent stem cells5. Therefore, the ability to rapidly and efficiently isolate undifferentiated hiPSCs from the parental somatic cells, feeder-layer cells, and spontaneously differentiated cells is a crucial step that remains a bottleneck in all human pluripotent stem cell research.
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Adhesive Signature-Based, Label-Free Isolation of Human Pluripotent Stem Cells
Ankur Singh,
Ankur Singh
Georgia Institute of Technology, Atlanta, GA
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Shalu Suri,
Shalu Suri
Georgia Institute of Technology, Atlanta, GA
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Ted T. Lee,
Ted T. Lee
Georgia Institute of Technology, Atlanta, GA
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Jamie M. Chilton,
Jamie M. Chilton
ArunA Biomedical, Inc., Athens, GA
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Steve L. Stice,
Steve L. Stice
ArunA Biomedical, Inc., Athens, GA
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Hang Lu,
Hang Lu
Georgia Institute of Technology, Atlanta, GA
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Todd C. McDevitt,
Todd C. McDevitt
Georgia Institute of Technology, Atlanta, GA
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Andrés J. Garcia
Andrés J. Garcia
Georgia Institute of Technology, Atlanta, GA
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Ankur Singh
Georgia Institute of Technology, Atlanta, GA
Shalu Suri
Georgia Institute of Technology, Atlanta, GA
Ted T. Lee
Georgia Institute of Technology, Atlanta, GA
Jamie M. Chilton
ArunA Biomedical, Inc., Athens, GA
Steve L. Stice
ArunA Biomedical, Inc., Athens, GA
Hang Lu
Georgia Institute of Technology, Atlanta, GA
Todd C. McDevitt
Georgia Institute of Technology, Atlanta, GA
Andrés J. Garcia
Georgia Institute of Technology, Atlanta, GA
Paper No:
SBC2012-80044, pp. 313-314; 2 pages
Published Online:
July 19, 2013
Citation
Singh, A, Suri, S, Lee, TT, Chilton, JM, Stice, SL, Lu, H, McDevitt, TC, & Garcia, AJ. "Adhesive Signature-Based, Label-Free Isolation of Human Pluripotent Stem Cells." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 313-314. ASME. https://doi.org/10.1115/SBC2012-80044
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