The use of sessile droplets in desiccation protocols has been brought into question due to potential inhomogeneity within the samples [1]. The moisture content and the corresponding viability that gets recorded, in most viability studies, represents that of the whole sample and does not account for any variation that may be present within the drop. One reason that trehalose is added to desiccation media as a biopreservative is to create a glassy state around the cell at certain moisture contents [2]. This makes it important to know the local residual moisture contents to better analyze viability results and whether or not the cells are actually capable of reaching a glassy state.

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