Human embryonic stem cells (hESCs) have remarkable potentials for breakthroughs in future cell-based therapeutics owing to their self-renewal capability and pluripotency [1–2]. However, their intrinsic mechanosensitivity to biophysical signals from the local cellular microenvironment is not well characterized [3–4]. In this work, we introduced a simple, yet precise, microfabrication strategy for accurate control and patterning of local nanoroughness on glass surfaces using photolithography and reactive ion etching (RIE). Our results demonstrated that nanoscale topological features could provide a potent regulatory signal over a diverse array of hESC behaviors, including their morphology, adhesion, proliferation and clonal expansion, and differentiation.
- Bioengineering Division
Nanotopography Directs Fate of Human Embryonic Stem Cells
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Chen, W, Villa-Diaz, LG, Sun, Y, Weng, S, Kim, JK, Krebsbach, PH, & Fu, J. "Nanotopography Directs Fate of Human Embryonic Stem Cells." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 1293-1294. ASME. https://doi.org/10.1115/SBC2012-80222
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