A large number of studies in cryobiology have focused on understanding the underlying biophysics at the cellular level to help predict survival outcome after cryopreservation or cryosurgery. While this behavior is increasingly well studied and understood in cells gaps remain in our understanding of how cells in tissues behave which can hamper freezing applications in tissues. This study compares freezing behavior in cells in suspension vs. attached (a model tissue) state to investigate any differences in cellular behavior in these two states.

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