Urodele amphibians like the newt, are able to completely regenerate lost organs and appendages without scarring. Differentiated tissues are considered a reservoir for uncommitted blastema cells that participate in the regeneration of the lost structure. To determine the influence of the extracellular matrix (ECM) on the recruitment of progenitor cells from the skeletal muscle, we immunohistochemically mapped the limb in 3D and found that a transitional ECM rich in hyaluronic acid (HA), tenascin-C (TN) and fibronectin (FN) is dynamically expressed during the early stages of regeneration [1]. Functional in vitro testing of different ECM components on primary muscle cells revealed that HA and TN support myoblast migration, inhibit differentiation and enhance the fragmentation of multinucleate myotubes and production of viable mononucleate myoblasts, cellular behaviors necessary for blastema formation [1]. In contrast, myoblasts plated on matrices that mimic ECM around differentiated muscle (FN, Matrigel and laminin) induced both proliferation and fusion.
Skip Nav Destination
ASME 2011 Summer Bioengineering Conference
June 22–25, 2011
Farmington, Pennsylvania, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-5458-7
PROCEEDINGS PAPER
The Mechanical and Biochemical Environment Controls Cellular Differentiation During Muscle Regeneration
Sarah Calve,
Sarah Calve
Northwestern University, Chicago, IL
Search for other works by this author on:
Hans-Georg Simon
Hans-Georg Simon
Northwestern University, Chicago, IL
Search for other works by this author on:
Sarah Calve
Northwestern University, Chicago, IL
Hans-Georg Simon
Northwestern University, Chicago, IL
Paper No:
SBC2011-53767, pp. 365-366; 2 pages
Published Online:
July 17, 2013
Citation
Calve, S, & Simon, H. "The Mechanical and Biochemical Environment Controls Cellular Differentiation During Muscle Regeneration." Proceedings of the ASME 2011 Summer Bioengineering Conference. ASME 2011 Summer Bioengineering Conference, Parts A and B. Farmington, Pennsylvania, USA. June 22–25, 2011. pp. 365-366. ASME. https://doi.org/10.1115/SBC2011-53767
Download citation file:
6
Views
Related Proceedings Papers
Related Articles
Design of an Endoreactor for the Cultivation of a Joint-Like-Structure
J. Med. Devices (June,2009)
A Magnetic Resonance-Compatible Loading Device for Dynamically Imaging Shortening and Lengthening Muscle Contraction Mechanics
J. Med. Devices (September,2009)
A New Fundamental Bioheat Equation for Muscle Tissue—Part II: Temperature of SAV Vessels
J Biomech Eng (February,2002)
Related Chapters
Identification of Dispersive Clays in the Texas Gulf Coast Area
Dispersive Clays, Related Piping, and Erosion in Geotechnical Projects
Chapter 5 | Clinical Perspectives on the Use of Allogenic Tissue Substitutes
Bone Graft Substitutes and Bone Regenerative Engineering
Ultrasound and Lipid-Coated Microbubbles Effect on Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells in 3D Printed Tissue Scaffold
Proceedings of the 10th International Symposium on Cavitation (CAV2018)