A wound healing assay is simple but effective method to study cell migration in vitro. Cell migration in vitro was found to mimic migration in vivo to some extent [1,2]. In wound healing assays, a “wound” is created by either scraping or mechanically crushing cells in a monolayer, thereby forming a denuded area. Cells migrate into the denuded area to complete coverage, and thereby “heal” the wound. Micrographs at regular time intervals are captured during such experiments for analysis of the process of migration.
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A Method for Quantitative Analysis of the Kinematics of Fibroblast Migration in a Monolayer Wound Model
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Topman, G, Sharabani-Yosef, O, & Gefen, A. "A Method for Quantitative Analysis of the Kinematics of Fibroblast Migration in a Monolayer Wound Model." Proceedings of the ASME 2011 Summer Bioengineering Conference. ASME 2011 Summer Bioengineering Conference, Parts A and B. Farmington, Pennsylvania, USA. June 22–25, 2011. pp. 355-356. ASME. https://doi.org/10.1115/SBC2011-53070
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