Changes in mechanical properties within brain tissues after losses in cell viability have not been well investigated. Lack of oxygen and nutrient transport can induce hypoxic neuronal injury and increase cell membrane permeability, and cell membranes and matrix components can lose their structural and mechanical integrity. These physical changes may have an effect on mechanical properties of brain tissue [1]. In this study, the viscoelastic behavior of two anatomical regions (cerebral cortex and hippocampus) in acute rat brain tissue slices were measured as a function of cell viability using indentation combined with optical coherence tomography (OCT). Neuronal viability in brain tissue slices was determined by measuring Fluoro-Jade C (FJC) staining to assay neuronal death or degeneration as a function of incubation time. OCT-measured deformation depths were compared with finite element (FE) simulations to estimate the relaxation of shear modulus. Measured equilibrium shear modulus (μ∞) after 8 hrs incubation was lower than μ∞ measured after 2 hrs incubation in the cerebral cortex (μ∞, 2hrs = 225 Pa, μ∞, 8hrs = 62 Pa) and hippocampus regions (μ∞, 2hrs = 170 Pa, μ∞, 8hrs = 33 Pa). Instantaneous shear modulus (μ0) after 8 hrs incubation was also an order of magnitude lower than μ0 after 2 hrs incubation in cortex (μ0, 2hrs = 1600 Pa, μ0, 8hrs = 100 Pa) and hippocampus regions (μ0, 2hrs = 370 Pa, μ0, 8hrs = 70 Pa). The results of this study provide a timeline for measuring mechanical properties of brain tissues ex vivo and provide better understanding of changes in brain modulus after injury or cell death.
Skip Nav Destination
ASME 2011 Summer Bioengineering Conference
June 22–25, 2011
Farmington, Pennsylvania, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-5458-7
PROCEEDINGS PAPER
Viscoelastic Property Changes of Acute Rat Brain Tissue Slices as a Function of Cell Viability
Sung Jin Lee,
Sung Jin Lee
University of Florida, Gainesville, FL
Search for other works by this author on:
Jingjing Sun,
Jingjing Sun
University of Florida, Gainesville, FL
Search for other works by this author on:
Michael King,
Michael King
University of Florida, Gainesville, FL
Search for other works by this author on:
Huikai Xie,
Huikai Xie
University of Florida, Gainesville, FL
Search for other works by this author on:
Malisa Sarntinoranont
Malisa Sarntinoranont
University of Florida, Gainesville, FL
Search for other works by this author on:
Sung Jin Lee
University of Florida, Gainesville, FL
Jingjing Sun
University of Florida, Gainesville, FL
Michael King
University of Florida, Gainesville, FL
Huikai Xie
University of Florida, Gainesville, FL
Malisa Sarntinoranont
University of Florida, Gainesville, FL
Paper No:
SBC2011-53909, pp. 1347-1348; 2 pages
Published Online:
July 17, 2013
Citation
Lee, SJ, Sun, J, King, M, Xie, H, & Sarntinoranont, M. "Viscoelastic Property Changes of Acute Rat Brain Tissue Slices as a Function of Cell Viability." Proceedings of the ASME 2011 Summer Bioengineering Conference. ASME 2011 Summer Bioengineering Conference, Parts A and B. Farmington, Pennsylvania, USA. June 22–25, 2011. pp. 1347-1348. ASME. https://doi.org/10.1115/SBC2011-53909
Download citation file:
6
Views
Related Proceedings Papers
Related Articles
Dynamic, Regional Mechanical Properties of the Porcine Brain: Indentation in the Coronal Plane
J Biomech Eng (July,2011)
Viscoelastic Properties of the P17 and Adult Rat Brain From Indentation in the Coronal Plane
J Biomech Eng (November,2013)
Modified Bilston Nonlinear Viscoelastic Model for Finite Element Head Injury Studies
J Biomech Eng (October,2006)
Related Chapters
Compressive Sensing Based Holographic Microwave Imaging
Electromagnetic Induction Imaging: Theory and Biomedical Applications
Processing/Structure/Properties Relationships in Polymer Blends for the Development of Functional Polymer Foams
Advances in Multidisciplinary Engineering
Characterization of Tissue Viscoelasticity from Shear Wave Speed Dispersion
Biomedical Applications of Vibration and Acoustics in Imaging and Characterizations