The urothelium is the epithelial lining of the ureters, urinary bladder, and urethra. Recent discoveries have suggested that in addition to providing a barrier function to urine, the urothelium actively participates in sensory functions related to thermal, chemical, and mechanical stimuli, and releases chemical signals in response[1]. In addition to a sensitivity to cell membrane stretch caused by wall tension upon bladder filling, in vitro studies by our group have shown that urothelial cells may be sensitive to hydrostatic pressure directly without requiring membrane stretching [2]. Specifically, primary cultures of rat bladder urothelial cells exposed to 10 cmH2O pressure on rigid substrates released significantly greater amounts of ATP compared to the baseline control without exposure to pressure. Moreover, this ATP response by rat urothelial cells to pressure was inhibited by pre-treatment of cells with ruthenium red, a non-specific antagonist of transient receptor potential (TRP) channels, suggesting a potential involvement of these channels in pressure mechanotransduction. Further understanding of the mechanisms, however, is needed to improve treatment of bladder dysfunction such as overactive bladder.
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ASME 2011 Summer Bioengineering Conference
June 22–25, 2011
Farmington, Pennsylvania, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-5458-7
PROCEEDINGS PAPER
Electrophysiological Investigation of Hydrostatic Pressure Mechanotransduction by Urothelial Cell Lines Available to Purchase
Kevin D. Champaigne,
Kevin D. Champaigne
Clemson University, Clemson, SC
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Sarette N. Jenderny,
Sarette N. Jenderny
Clemson University, Clemson, SC
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Jiro Nagatomi
Jiro Nagatomi
Clemson University, Clemson, SC
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Kevin D. Champaigne
Clemson University, Clemson, SC
Sarette N. Jenderny
Clemson University, Clemson, SC
Jiro Nagatomi
Clemson University, Clemson, SC
Paper No:
SBC2011-53518, pp. 1239-1240; 2 pages
Published Online:
July 17, 2013
Citation
Champaigne, KD, Jenderny, SN, & Nagatomi, J. "Electrophysiological Investigation of Hydrostatic Pressure Mechanotransduction by Urothelial Cell Lines." Proceedings of the ASME 2011 Summer Bioengineering Conference. ASME 2011 Summer Bioengineering Conference, Parts A and B. Farmington, Pennsylvania, USA. June 22–25, 2011. pp. 1239-1240. ASME. https://doi.org/10.1115/SBC2011-53518
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