Cryo-preservation of precious cells like stem cells and oocytes requires high success rate because they are hard to obtain and limited in number. Simulation of the freezing process could: (1)Serve as an inexpensive replacement for vivo experiments (when collecting complete experiment data is too costly or time consuming); (2) Help optimize the cyro-preservation protocols. (3) Help to confirm or disprove intracellular ice formation theories. Most of the published models simulating the water loss and intracellular ice formation have treated the cell as a uniform volume with evenly distributed water content. Albeit being a well-founded premise given consideration to the minuteness size of the cell, there are several issues it cannot properly address.

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