Embryonic stem cell derived cardiomyocytes are deemed an attractive treatment option for myocardial infarction. Their clinical efficacy, however, has not been unequivocally demonstrated. There is a need for better understanding and characterization of the cardiogenesis process. A microfluidic platform in vitro is used to dissect and better understand the differentiation process. Through this study, we find that while embryoid bodies (EBs) flatten out in a well plate system, differentiated EBs self-assemble into complex 3D structures. The beating regions of EBs are also different. Most beating areas are observed in a ring pattern on 2D well plates around the center, self-assembled beating large 3D aggregates are found in microfluidic devices. Furthermore, inspired by the natural mechanical environment of the heart, we applied uniaxial cyclic mechanical stretch to EBs. Results suggest that prolonged mechanical stimulation acts as a negative regulator of cardiogenesis. From this study, we conclude that the culture environments can influence differentiation of embryonic stem cells into cardiomycytes, and that the use of microfluidic systems can provide new insights into the differentiation process.
Skip Nav Destination
ASME 2009 Summer Bioengineering Conference
June 17–21, 2009
Lake Tahoe, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4891-3
PROCEEDINGS PAPER
Induction of Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells in a Confined Microfluidic Environment Available to Purchase
Chen-rei Wan,
Chen-rei Wan
Massachusetts Institute of Technology, Cambridge, MA
Search for other works by this author on:
Seok Chung,
Seok Chung
Massachusetts Institute of Technology, Cambridge, MA
Search for other works by this author on:
Ryo Sudo,
Ryo Sudo
Massachusetts Institute of Technology, Cambridge, MA
Search for other works by this author on:
Roger D. Kamm
Roger D. Kamm
Massachusetts Institute of Technology, Cambridge, MA
Search for other works by this author on:
Chen-rei Wan
Massachusetts Institute of Technology, Cambridge, MA
Seok Chung
Massachusetts Institute of Technology, Cambridge, MA
Ryo Sudo
Massachusetts Institute of Technology, Cambridge, MA
Roger D. Kamm
Massachusetts Institute of Technology, Cambridge, MA
Paper No:
SBC2009-203995, pp. 917-918; 2 pages
Published Online:
July 19, 2013
Citation
Wan, C, Chung, S, Sudo, R, & Kamm, RD. "Induction of Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells in a Confined Microfluidic Environment." Proceedings of the ASME 2009 Summer Bioengineering Conference. ASME 2009 Summer Bioengineering Conference, Parts A and B. Lake Tahoe, California, USA. June 17–21, 2009. pp. 917-918. ASME. https://doi.org/10.1115/SBC2009-203995
Download citation file:
5
Views
Related Proceedings Papers
Related Articles
Editorial
J. Med. Devices (March,2025)
Self-Assembled Axisymmetric Microscale Periodic Wrinkles on Elastomer Fibers
J. Micro Nano-Manuf (June,2017)
Comparison of Geometries for Diffusion-Based Extraction of Dimethyl Sulphoxide From a Cell Suspension
J. Med. Devices (June,2008)
Related Chapters
Chapter 5 | Clinical Perspectives on the Use of Allogenic Tissue Substitutes
Bone Graft Substitutes and Bone Regenerative Engineering
Ultrasound and Lipid-Coated Microbubbles Effect on Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells in 3D Printed Tissue Scaffold
Proceedings of the 10th International Symposium on Cavitation (CAV2018)
Introduction
Bacteriophage T4 Tail Fibers as a Basis for Structured Assemblies