Endothelial cells line all blood vessels and regulate many homeostatic functions (e.g. platelet aggregation, vascular tone, vascular cell proliferation, leukocyte adhesion) by production of the signaling molecule nitric oxide (NO). NO bioavailability and thus endothelial cell function are compromised in many chronic disease states, including diabetes mellitus and its associated micro- and macrovascular complications (e.g. impaired wound healing and atherosclerosis, respectively) [1]. In the specific case of diabetic wound healing, application of exogenous NO to the diseased tissue may help restore critical NO-mediated processes and could positively impact healing and overall patient health [2]. We have developed a novel pin-to-hole spark discharge plasma device that generates NO and can be applied to cultured endothelial cells with minimal cell injury or death. We propose that this plasma device represents a promising novel method for topical NO application.
Skip Nav Destination
ASME 2009 Summer Bioengineering Conference
June 17–21, 2009
Lake Tahoe, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4891-3
PROCEEDINGS PAPER
A Pin-to-Hole Spark Discharge Plasma Generates Nitric Oxide and Can Be Safely Applied to an Endothelial Cell Monolayer
Drew A. Graham,
Drew A. Graham
Drexel University, Philadelphia, PA
Search for other works by this author on:
Danil V. Dobrynin,
Danil V. Dobrynin
Drexel University, Philadelphia, PA
Search for other works by this author on:
Alexander Fridman,
Alexander Fridman
Drexel University, Philadelphia, PA
Search for other works by this author on:
Gary Friedman,
Gary Friedman
Drexel University, Philadelphia, PA
Search for other works by this author on:
Alisa Morss Clyne
Alisa Morss Clyne
Drexel University, Philadelphia, PA
Search for other works by this author on:
Drew A. Graham
Drexel University, Philadelphia, PA
Danil V. Dobrynin
Drexel University, Philadelphia, PA
Alexander Fridman
Drexel University, Philadelphia, PA
Gary Friedman
Drexel University, Philadelphia, PA
Alisa Morss Clyne
Drexel University, Philadelphia, PA
Paper No:
SBC2009-206764, pp. 163-164; 2 pages
Published Online:
July 19, 2013
Citation
Graham, DA, Dobrynin, DV, Fridman, A, Friedman, G, & Clyne, AM. "A Pin-to-Hole Spark Discharge Plasma Generates Nitric Oxide and Can Be Safely Applied to an Endothelial Cell Monolayer." Proceedings of the ASME 2009 Summer Bioengineering Conference. ASME 2009 Summer Bioengineering Conference, Parts A and B. Lake Tahoe, California, USA. June 17–21, 2009. pp. 163-164. ASME. https://doi.org/10.1115/SBC2009-206764
Download citation file:
6
Views
0
Citations
Related Proceedings Papers
Related Articles
Vascular Cell Adhesion Molecule-1 Expression in Endothelial Cells Exposed to Physiological Coronary Wall Shear Stresses
J Biomech Eng (August,2009)
CT Visualization of Cryoablation in Pulmonary Veins
J. Med. Devices (June,2009)
Related Chapters
Introduction
Mechanical Blood Trauma in Circulatory-Assist Devices
Introduction and scope
Impedimetric Biosensors for Medical Applications: Current Progress and Challenges
Clinical issues and experience
Mechanical Blood Trauma in Circulatory-Assist Devices