It is becoming increasingly evident, that of the signaling modalities relevant to the cell-extracellular matrix (ECM) microenvironment, the mechanical component is a very important mediator of cell behavior (reviewed in [1, 2]). Indeed, proliferation, ECM protein expression (collagen), matrix metalloproteinase (MMP) levels, migration, and stem cell differentiation, have all been shown to be affected by mechanical environmental cues [3, 4]. Although the importance of physical signaling mechanisms has been well established, the bulk of this work has yet to be translated to a more physiologic 3D microenvironment [1]. Self-assembling collagen matrices provide a biochemically, biophysically relevant 3D model of soft tissues in which biomechanical studies can be performed [5, 6]. It is with this 3D tissue model in mind, that a biaxial mechanical testing system (BMTS) was devised, built, tested, and applied to the study of cell-ECM biomechanics. The completion of this device has enabled us, to undertake a multi-scale, multidimensional study of cell-ECM mechanics. Hierarchical quantification of cell and ECM strains using digital image correlation (DIC) facilitate a more complete understanding of the mechanical response of cells to macroscopic loads and deformations. Furthermore, transfection of cells with GFP tagged actin binding protein utrophin (UTR-GFP) enables qualitative assessment of cytoskeletal deformations [7].
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ASME 2009 Summer Bioengineering Conference
June 17–21, 2009
Lake Tahoe, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4891-3
PROCEEDINGS PAPER
Multiaxial Study of Fibroblast Biomechanics in a 3D Collagen Matrix
Brett J. Bell,
Brett J. Bell
Purdue University, West Lafayette, IN
Search for other works by this author on:
Sherry L. Voytik-Harbin
Sherry L. Voytik-Harbin
Purdue University, West Lafayette, IN
Search for other works by this author on:
Brett J. Bell
Purdue University, West Lafayette, IN
Sherry L. Voytik-Harbin
Purdue University, West Lafayette, IN
Paper No:
SBC2009-206722, pp. 1131-1132; 2 pages
Published Online:
July 19, 2013
Citation
Bell, BJ, & Voytik-Harbin, SL. "Multiaxial Study of Fibroblast Biomechanics in a 3D Collagen Matrix." Proceedings of the ASME 2009 Summer Bioengineering Conference. ASME 2009 Summer Bioengineering Conference, Parts A and B. Lake Tahoe, California, USA. June 17–21, 2009. pp. 1131-1132. ASME. https://doi.org/10.1115/SBC2009-206722
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