Endothelial cells (ECs) form the inner lining of the blood vasculature and are exposed to shear stress (τ), the tangential component of hemodynamic forces. ECs transduce τ into biochemical signals possibly via EC-membrane perturbations. We have previously used confocal-FRAP on the DiI-stained plasma membranes of confluent cultured bovine aortic ECs (BAECs) to show that τ induces a rapid, spatially heterogeneous, and time-dependent increase in the lateral diffusion of the fluorescent lipoid probe in the BAEC membrane [1]. We now present evidence at the single molecule level that shear stress differentially perturbs membrane domains that are defined by their selective staining by lipoid dyes (DiI) of differing alkyl chain lengths. This study is the first to directly measure perturbation by shear stress of endothelial cell membrane microdomains.
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ASME 2009 Summer Bioengineering Conference
June 17–21, 2009
Lake Tahoe, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4891-3
PROCEEDINGS PAPER
Shear Stress Induces Time- and Domain-Dependent Changes in Lipid Dynamics of Endothelial Cell Membranes
Tristan Tabouillot,
Tristan Tabouillot
Pennsylvania State University, University Park, PA
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Hari S. Muddana,
Hari S. Muddana
Pennsylvania State University, University Park, PA
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Peter J. Butler
Peter J. Butler
Pennsylvania State University, University Park, PA
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Tristan Tabouillot
Pennsylvania State University, University Park, PA
Hari S. Muddana
Pennsylvania State University, University Park, PA
Peter J. Butler
Pennsylvania State University, University Park, PA
Paper No:
SBC2009-206882, pp. 105-106; 2 pages
Published Online:
July 19, 2013
Citation
Tabouillot, T, Muddana, HS, & Butler, PJ. "Shear Stress Induces Time- and Domain-Dependent Changes in Lipid Dynamics of Endothelial Cell Membranes." Proceedings of the ASME 2009 Summer Bioengineering Conference. ASME 2009 Summer Bioengineering Conference, Parts A and B. Lake Tahoe, California, USA. June 17–21, 2009. pp. 105-106. ASME. https://doi.org/10.1115/SBC2009-206882
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