Quantitation of Laminin Adsorbed Onto Polydimethylsiloxane Surfaces Using Various Treatment Protocols

There is considerable interest in how cells respond to mechanical stimuli, from the ligands used to transmit the stimulus to the signaling pathways initiated and the proteins expressed upon phenotype change [1]. Previous work focused on the evaluation of the quality of the extracellular matrix (ECM) coating and cell proliferation [2]. Our focus is the characterization of a flexible polymeric substrate, treated with ECM, used to induce tensile strain on cells. In this work, we expand our physical characterization of the protein modified polydimethylsiloxane (PDMS) surface by quantifying the coverage of laminin on PDMS, plasma-treated PDMS, and PDMS treated with plasma and aminopropyltrimethoxysilane (APTMS) (Silane_70 protocol) using X-ray reflectivity.