Applications involving freeze-thaw in arteries such as cryoplasty and cryopreservation alter the arterial biomechanics significantly [1]. Tissue dehydration or bulk water loss is observed following freeze-thaw in native arteries as well as other artificial tissues [1, 2]. It is hypothesized that tissue dehydration observed during freeze-thaw is an important mechanism underlying the biomechanical changes in arteries. In order to test this hypothesis, dehydration was induced in arteries (without changing temperature or phase) by treating them with different concentrations of hyperosmotic mannitol solutions. Changes to smooth muscle cell (SMC) contractility, collagen matrix structure and overall artery biomechanics were studied following tissue dehydration. SMC contractility and relaxation were measured by studying the response of arteries to norepinephrine (NE) and acetylcholine (AC) respectively. Collagen matrix structure was assessed by studying the thermal denaturation of collagen due to heating using Fourier transform infrared (FTIR) spectroscopy and the overall artery biomechanics through uniaxial tensile tests.
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ASME 2008 Summer Bioengineering Conference
June 25–29, 2008
Marco Island, Florida, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4321-5
PROCEEDINGS PAPER
Effect of Tissue Dehydration on Smooth Muscle Cell Contractility, Collagen Matrix Structure and Overall Artery Biomechanics
Ramji Venkatasubramanian,
Ramji Venkatasubramanian
University of Minnesota, Minneapolis, MN
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Wim Wolkers,
Wim Wolkers
University of Minnesota, Minneapolis, MN
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Charles Soule,
Charles Soule
University of Minnesota, Minneapolis, MN
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Paul Iaizzo,
Paul Iaizzo
University of Minnesota, Minneapolis, MN
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John Bischof
John Bischof
University of Minnesota, Minneapolis, MN
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Ramji Venkatasubramanian
University of Minnesota, Minneapolis, MN
Wim Wolkers
University of Minnesota, Minneapolis, MN
Charles Soule
University of Minnesota, Minneapolis, MN
Paul Iaizzo
University of Minnesota, Minneapolis, MN
John Bischof
University of Minnesota, Minneapolis, MN
Paper No:
SBC2008-192346, pp. 261-262; 2 pages
Published Online:
March 13, 2014
Citation
Venkatasubramanian, R, Wolkers, W, Soule, C, Iaizzo, P, & Bischof, J. "Effect of Tissue Dehydration on Smooth Muscle Cell Contractility, Collagen Matrix Structure and Overall Artery Biomechanics." Proceedings of the ASME 2008 Summer Bioengineering Conference. ASME 2008 Summer Bioengineering Conference, Parts A and B. Marco Island, Florida, USA. June 25–29, 2008. pp. 261-262. ASME. https://doi.org/10.1115/SBC2008-192346
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