Measurement of the mechanical properties of bone is important for estimation of the local mechanical response of bone cells to loading experienced on a larger scale. An increasing number of measurements of the hardness and Young’s modulus of bone tissue have been undertaken using nanoindentation [1,2]. However, testing conditions have not been uniform. The interactions that can occur between testing condition parameters were considered in this study, and average hardness and Young’s modulus were obtained as a function of indentation creep testing conditions (maximum load, loading/unloading rate (both equal in magnitude), load-holding time, and indenter shape).

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