An integrated opto-electric biosensor is developed that uses an optically transparent and electrically conductive indium tin oxide (ITO) thin film coated on a slide glass substrate. This biosensor can simultaneously acquire the micro-impedance response and microscopic images of live cells in vitro under various toxic agent stimuli. The dynamic response of live porcine pulmonary artery endothelial cells (PPAECs) exposed to various doses of cytochalasin D are comprehensively examined by monitoring the micro-impedance characteristics at a specified frequency and DICM images using the opto-electric biosensor. The change in PPAEC morphology and motility caused by cytochalasin D clearly illustrates the dose-dependent actin filament disruption where optical images are correlated with the changes in the micro-electric impedance.
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ASME 2007 Summer Bioengineering Conference
June 20–24, 2007
Keystone, Colorado, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-4798-5
PROCEEDINGS PAPER
Opto-Electric Biosensor to Examine In Vitro Toxicity Stimuli to Endothelial Cell Motility and Morphology Available to Purchase
Chang K. Choi,
Chang K. Choi
The University of Tennessee, Knoxville, TN
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Chuck Margraves,
Chuck Margraves
The University of Tennessee, Knoxville, TN
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Anthony E. English,
Anthony E. English
The University of Tennessee, Knoxville, TN
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Kenneth D. Kihm
Kenneth D. Kihm
The University of Tennessee, Knoxville, TN
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Chang K. Choi
The University of Tennessee, Knoxville, TN
Chuck Margraves
The University of Tennessee, Knoxville, TN
Anthony E. English
The University of Tennessee, Knoxville, TN
Kenneth D. Kihm
The University of Tennessee, Knoxville, TN
Paper No:
SBC2007-176715, pp. 61-62; 2 pages
Published Online:
March 12, 2014
Citation
Choi, CK, Margraves, C, English, AE, & Kihm, KD. "Opto-Electric Biosensor to Examine In Vitro Toxicity Stimuli to Endothelial Cell Motility and Morphology." Proceedings of the ASME 2007 Summer Bioengineering Conference. ASME 2007 Summer Bioengineering Conference. Keystone, Colorado, USA. June 20–24, 2007. pp. 61-62. ASME. https://doi.org/10.1115/SBC2007-176715
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