Tissue engineering offers an attractive alternative to direct repair or reconstruction of injuries to tendons, ligaments and capsular structures that represent almost 45% of the 32 million musculoskeletal injuries that occur each year in the United States [1]. Mesenchymal stem cell (MSC)-seeded collagen constructs are currently being used by our group to repair tendon injuries in the rabbit model [2, 3]. Although these cell-assisted repairs exhibit 50% greater maximum force and stiffness at 12 weeks compared to values for natural repair, tissues often lack the maximum force sufficient to resist the peak in vivo forces acting on the repair site [3]. Our laboratory has previously demonstrated that in vitro construct stiffness and repair stiffness at 12 weeks post surgery are positively correlated [4]. Therefore, in an effort to further improve the repair outcome using tissue engineering, we continue our investigation of scaffold materials to create stiffer MSC-collagen constructs. Our group has recently evaluated two scaffold materials, type I collagen sponges fabricated within the Engineered Skin Lab (ESL, Shriners Hospitals for Children) by a freezing and lyophilization process with and without glycosaminoglycan (chondroitin-6-sulfate; GAG) [5] and found the ESL sponges to significantly improve biomechanical properties of the constructs compared to sponges we currently use in the lab (P1076, Kensey Nash Corporation, Exton, PA). This study also demonstrated that GAG significantly upregulates collagen type I, decorin, and fibronectin gene expression (unpublished results).
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ASME 2007 Summer Bioengineering Conference
June 20–24, 2007
Keystone, Colorado, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-4798-5
PROCEEDINGS PAPER
Combined Effect of Glycosaminoglycan and Mechanical Stimulation on the In Vitro Biomechanics of Tissue Engineered Tendon Constructs
Kirsten R. C. Kinneberg,
Kirsten R. C. Kinneberg
University of Cincinnati, Cincinnati, OH
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Victor S. Nirmalanandhan,
Victor S. Nirmalanandhan
University of Cincinnati, Cincinnati, OH
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Heather M. Powell,
Heather M. Powell
Shriners Hospitals for Children, Cincinnati, OH
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Steven T. Boyce,
Steven T. Boyce
Shriners Hospitals for Children, Cincinnati, OH
University of Cincinnati, Cincinnati, OH
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David L. Butler
David L. Butler
University of Cincinnati, Cincinnati, OH
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Kirsten R. C. Kinneberg
University of Cincinnati, Cincinnati, OH
Victor S. Nirmalanandhan
University of Cincinnati, Cincinnati, OH
Heather M. Powell
Shriners Hospitals for Children, Cincinnati, OH
Steven T. Boyce
Shriners Hospitals for Children, Cincinnati, OH
University of Cincinnati, Cincinnati, OH
David L. Butler
University of Cincinnati, Cincinnati, OH
Paper No:
SBC2007-176386, pp. 1007-1008; 2 pages
Published Online:
March 12, 2014
Citation
Kinneberg, KRC, Nirmalanandhan, VS, Powell, HM, Boyce, ST, & Butler, DL. "Combined Effect of Glycosaminoglycan and Mechanical Stimulation on the In Vitro Biomechanics of Tissue Engineered Tendon Constructs." Proceedings of the ASME 2007 Summer Bioengineering Conference. ASME 2007 Summer Bioengineering Conference. Keystone, Colorado, USA. June 20–24, 2007. pp. 1007-1008. ASME. https://doi.org/10.1115/SBC2007-176386
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