Microstructure evolution and creep rupture behavior of modified 9Cr-1Mo steel welded joint used for steam cooler in high pressure heater system were systematically studied in this paper. Creep tests were carried out using uniaxial creep specimens machined from the normalized and tempered plate at 818K and 838K with the stresses ranging from 150 to 225MPa. The curve of stress vs. rupture time was achieved to evaluate the creep rupture life of modified 9Cr-1Mo steel welded joint. The creep data were analyzed in terms of Norton’s power law, Monkman-Grant relation and modified Monkman-Grant relation. Microstructure before and after creep exposure were analyzed by optical microscope, scanning electron microscope and transmission electron microscope, to further explain the rupture mechanisms of modified 9Cr-1Mo steel welded joint.