Quenched and tempered high strength steel 07MnMoVR with a better combination of mechanical properties and low susceptibility to welding crack has attracted attention for applications in engineering fields. Exposure to fire will subject steel to thermally induced environmental conditions that may alter the material’s properties. The residual strength after a fire event is important to assess the extent of the fire damage and the potential reusability of the vessel. This paper presents the details of an experimental investigation on the post-fire mechanical properties of 07MnMoVR steel. Uniaxial tension tests and Charpy impact tests were performed on coupons exposed to elevated temperatures varying from 550°C to 850°C for half an hour to 8 hours and then naturally cooled in air or cooled by water. The post-fire stress-strain curves, strength, ductility and impact toughness of 07MnMoVR steel are discussed. The results show that the yield plateau in post-fire stress-strain curves disappears when the exposure temperature is higher than 700°C. The residual yield strength and ultimate strength decrease firstly and increase afterward with increasing exposure temperature. The influences of duration time on the residual strength are considerable for exposure at 650°C. The post-fire impact toughness of 07MnMoVR steel at −20°C degrades drastically with increasing duration time when the exposure temperature reaches 700°C. The effects of cooling methods on strength and toughness become significant when the exposure temperature exceeds 750°C. The critical tempetature for the mechanical properties deterioration is 650°C. This study can provide basis data and guidelines for the fitness for service assessment of 07MnMoVR steel suffered from fire accident.

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