This research is intended to examine the amount of changes that can happen in material characteristics after retesting. Stress relaxation test is conducted on the same samples of the swine brain tissue for several times in small and large deformations. The mechanical properties of the substance are calculated before and after retest and the constants of the tissue, as mechanical characteristics, are determined and compared. Short- and long-term moduli, relaxation times and relaxation functions are of those data that are calculated and compared to understand how much they decay after repeating the experiments. The results show that applying different tests on one sample slightly changes the mechanical properties of the tissue and, as a result, it is partly possible to perform more than one test on the same sample resulting in less sample preparation, time and effort.

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