Inflammation plays a central role in the progression of disc degeneration, which is strongly implicated as a cause of low back pain. The objectives of this in vivo study were: first to investigate whether PLGA microspheres could be retained in the disc, and second to test whether IL-1ra delivered from those microspheres could effectively inhibit IL-1β induced extracellular matrix loss using a rat caudal disc model. Three caudal disc levels (C6–7 to C8–9) of 6 Sprague-Dawley rats were exposed via a dorsal midline incision for treatment. Fluorescently labeled microspheres were clearly visible in the disc at all time points except 8 weeks. The GAG content of discs injected with IL-1β alone was significantly lower than that of the intact controls and the microsphere treatment group. For discs injected with both IL-1β and IL-1ra microspheres, GAG content was not different from the intact control. In this study we demonstrated for the first time that IL-1ra delivered from microspheres prevents IL-1β induced GAG loss in vivo, and that microspheres are retained in the disc space for at least 4 weeks.
- Nanotechnology Institute
- Bioengineering Division
In Vivo Delivery of IL-1ra From PLGA Microspheres Prevents IL-1β Induced Glycosaminoglycan Loss in the Rat Caudal Intervertebral Disc
Gorth, DJ, Martin, JT, Dodge, GR, Hebela, NM, Malhotra, NR, Mauck, RL, Elliott, DM, & Smith, LJ. "In Vivo Delivery of IL-1ra From PLGA Microspheres Prevents IL-1β Induced Glycosaminoglycan Loss in the Rat Caudal Intervertebral Disc." Proceedings of the ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology. ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology. Boston, Massachusetts, USA. February 4–6, 2013. V001T04A004. ASME. https://doi.org/10.1115/NEMB2013-93134
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