Noninvasive radiofrequency (RF) fields heat metal nanoparticles in a concentration dependent fashion. Gold nanoparticles are especially interesting for biomedical applications because they not only heat well, but they have an established biosafety profile. Antibody-targeted gold nanoparticles have been used to induce hyperthermic cytotoxicity when exposed to RF fields. Two carcinoma cells lines, Panc-1 and Hep3B, were individually treated with 100 nM panitumumab and trastuzumab antibody conjugated 10 nm gold nanoparticles and subsequently exposed to an RF field for a total generator power of ∼100 kJ. Two days later, control cells treated with antibody labeled gold nanoparticles, but not exposed to the RF field, maintained an average viability of 92.1% ± 2.5% for Hep 3B cells and 89.1% ± 2.1% for Panc-1 cells based on flow cytometry. Panc-1 cells treated the same way with subsequent RF field exposure had viability less than 80% (p ∼ 0.001). Hep3B cells showed a similar decrease in viability after trastuzumab-gold treatment (74.5% ± 6.9%), but not panitumumab. This demonstrates a new and developing use of antibodies, specifically, against EGFR family targets.
Skip Nav Destination
ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
February 7–10, 2010
Houston, Texas, USA
Conference Sponsors:
- ASME Nanotechnology Council
ISBN:
978-0-7918-4392-5
PROCEEDINGS PAPER
Hyperthermic Cytotoxicity Induced by Noninvasive Radiofrequency Field Exposure After Delivery of EGFR Family Targeted Gold Nanoparticles
Evan S. Glazer,
Evan S. Glazer
University of Texas M. D. Anderson Cancer Center, Houston, TX
Search for other works by this author on:
Warna D. Kaluarachchi,
Warna D. Kaluarachchi
University of Texas M. D. Anderson Cancer Center, Houston, TX
Search for other works by this author on:
Steven A. Curley
Steven A. Curley
University of Texas M. D. Anderson Cancer Center, Houston, TX
Search for other works by this author on:
Evan S. Glazer
University of Texas M. D. Anderson Cancer Center, Houston, TX
Warna D. Kaluarachchi
University of Texas M. D. Anderson Cancer Center, Houston, TX
Steven A. Curley
University of Texas M. D. Anderson Cancer Center, Houston, TX
Paper No:
NEMB2010-13172, pp. 97-98; 2 pages
Published Online:
December 22, 2010
Citation
Glazer, ES, Kaluarachchi, WD, & Curley, SA. "Hyperthermic Cytotoxicity Induced by Noninvasive Radiofrequency Field Exposure After Delivery of EGFR Family Targeted Gold Nanoparticles." Proceedings of the ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. Houston, Texas, USA. February 7–10, 2010. pp. 97-98. ASME. https://doi.org/10.1115/NEMB2010-13172
Download citation file:
6
Views
Related Proceedings Papers
Related Articles
Solid-State Combustion of Metallic Nanoparticles: New Possibilities for an Alternative Energy Carrier
J. Energy Resour. Technol (March,2007)
Synthesis of Silica-Coated Iron Oxide Nanoparticles for Magnetic Resonance Contrast Enhancement and Thermal Therapies
J. Med. Devices (June,2011)
Magnetic Nanofilms for Biomedical Applications
J. Nanotechnol. Eng. Med (May,2010)
Related Chapters
Drug Delivery Nanosystems as a Promising Area of Modern Chemistry and Medicine. Silica Nanoparticles as Potential Drug Carriers
Silica Nanoparticles as Drug Delivery System for Immunomodulator GMDP (Biomedical & Nanomedical Technologies - Concise Monograph Series)
Conclusion
Biopolymers Based Micro- and Nano-Materials
Outlook
Closed-Cycle Gas Turbines: Operating Experience and Future Potential