The activity of proteins and their complexes often involves the conversion of chemical energy (stored or supplied) into mechanical work through conformational changes. Mechanical forces are also crucial for the regulation of the structure and function of cells and tissues. Thus, the shape of eukaryotic cells is the result of cycles of mechano-sensing, mechano-transduction, and mechano-response. Recently developed single-molecule atomic force microscopy (AFM) techniques can be used to manipulate single molecules, both in real time and under physiological conditions, and are ideally suited to directly quantify the forces involved in both intra- and intermolecular protein interactions. In combination with molecular biology and computer simulations, these techniques have been applied to characterize the unfolding and refolding reactions in a variety of proteins, such as titin (an elastic mechano-sensing protein found in muscle) and polycystin-1 (PC1, a mechanosensor found in the kidney).
Skip Nav Destination
ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
February 7–10, 2010
Houston, Texas, USA
Conference Sponsors:
- ASME Nanotechnology Council
ISBN:
978-0-7918-4392-5
PROCEEDINGS PAPER
Nanoscale Analysis of the Effect of Pathogenic Mutations on Polycystin-1 Available to Purchase
Liang Ma,
Liang Ma
University of Texas Medical Branch, Galveston, TX
Search for other works by this author on:
Meixiang Xu,
Meixiang Xu
University of Texas Medical Branch, Galveston, TX
Search for other works by this author on:
Andres F. Oberhauser
Andres F. Oberhauser
University of Texas Medical Branch, Galveston, TX
Search for other works by this author on:
Liang Ma
University of Texas Medical Branch, Galveston, TX
Meixiang Xu
University of Texas Medical Branch, Galveston, TX
Andres F. Oberhauser
University of Texas Medical Branch, Galveston, TX
Paper No:
NEMB2010-13093, pp. 365-366; 2 pages
Published Online:
December 22, 2010
Citation
Ma, L, Xu, M, & Oberhauser, AF. "Nanoscale Analysis of the Effect of Pathogenic Mutations on Polycystin-1." Proceedings of the ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. Houston, Texas, USA. February 7–10, 2010. pp. 365-366. ASME. https://doi.org/10.1115/NEMB2010-13093
Download citation file:
6
Views
Related Proceedings Papers
Related Articles
Age-Dependent Regional Mechanical Properties of the Rat Hippocampus and Cortex
J Biomech Eng (January,2010)
On a Relationship Between the Arrhenius Parameters from Thermal Damage Studies
J Biomech Eng (April,2003)
KTP High Power Laser-Tissue Interactions: In Vitro Experiment and Simulation
J. Med. Devices (June,2009)
Related Chapters
Experimental Studies
Nanoparticles and Brain Tumor Treatment
Characterization and evaluation
Biocompatible Nanomaterials for Targeted and Controlled Delivery of Biomacromolecules
Glioma Biology
Nanoparticles and Brain Tumor Treatment