Inkjet printing technique has been developed and applied in many areas. This rapid and simple technique can dispense small amount of selected material at intended location accurately. Due to these advantages, it has been applied to the field of biology such as tissue engineering and microbiology lately. We developed patterning methods based on inkjet printing technique employing bacteria, and generated two-dimensional bacterial cell array on the agar media using a commercially available thermal inkjet printer reformed partially. In this study, we aimed to apply the inkjet-printed bacterial cell array to biosensor. We measured the maximum resolution, accuracy and reproducibility of the bacterial array printed at 600 dpi. In addition, we were able to print three kinds of bacterial strains simultaneously using color cartridges which also enabling synchronous printing of both bacterial solution and known chemical. We applied this technique for studying the growth response of individual bacteria to different levels of stiffness, and the chemotactic response of bacterial colonies.

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