The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (∼sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.
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ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
February 7–10, 2010
Houston, Texas, USA
Conference Sponsors:
- ASME Nanotechnology Council
ISBN:
978-0-7918-4392-5
PROCEEDINGS PAPER
Ultra Sensitive Detection of Eneterovirus 71 by Modified Electrochemical Impedance Spectroscopy Available to Purchase
Shin-Hua Tseng,
Shin-Hua Tseng
National Chiao Tung University, Hsinchu, Taiwan
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Dion T. Tseng,
Dion T. Tseng
National Chiao Tung University, Hsinchu, Taiwan
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Tzu-Cheng Lee,
Tzu-Cheng Lee
National Chiao Tung University, Hsinchu, Taiwan
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Tsai-Mu Cheng,
Tsai-Mu Cheng
National Chiao Tung University, Hsinchu, Taiwan
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Jyh-Yuan Yang,
Jyh-Yuan Yang
Center for Disease Control, Taipei, Taiwan
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Ruo-Yu Hsieh,
Ruo-Yu Hsieh
Center for Disease Control, Taipei, Taiwan
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Chuan-Mei Tsai,
Chuan-Mei Tsai
National Nano Devices Laboratories, Hsinchu, Taiwann
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Chia-Ching Chang
Chia-Ching Chang
National Chiao Tung University; Academia Sinica, Taipei, Taiwann
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Shin-Hua Tseng
National Chiao Tung University, Hsinchu, Taiwan
Dion T. Tseng
National Chiao Tung University, Hsinchu, Taiwan
Tzu-Cheng Lee
National Chiao Tung University, Hsinchu, Taiwan
Tsai-Mu Cheng
National Chiao Tung University, Hsinchu, Taiwan
Jyh-Yuan Yang
Center for Disease Control, Taipei, Taiwan
Ruo-Yu Hsieh
Center for Disease Control, Taipei, Taiwan
Chuan-Mei Tsai
National Nano Devices Laboratories, Hsinchu, Taiwann
Chia-Ching Chang
National Chiao Tung University; Academia Sinica, Taipei, Taiwann
Paper No:
NEMB2010-13143, pp. 27-28; 2 pages
Published Online:
December 22, 2010
Citation
Tseng, S, Tseng, DT, Lee, T, Cheng, T, Yang, J, Hsieh, R, Tsai, C, & Chang, C. "Ultra Sensitive Detection of Eneterovirus 71 by Modified Electrochemical Impedance Spectroscopy." Proceedings of the ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. Houston, Texas, USA. February 7–10, 2010. pp. 27-28. ASME. https://doi.org/10.1115/NEMB2010-13143
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