The competence and compatibility of biomaterials is always challenging and demanding in biotech industries. Hydroxyapatite (HAp) is a useful biomaterial for biological applications due to its especial properties. In this paper, a dry mechanochemical process is introduced to produce hydroxyapatite nanoparticles. Structural and morphological properties of HAp powder are studied by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The results show that the single-crystal HAp nanospherical particles are successfully produced during milling process. Two different metallic and polymeric vials are applied and the results are compared for both vials. The results verify that the HAp nanoparticles are single crystal and their sizes are in the ranges of 12–24 nm.

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