We are investigating silicon-based platforms for detection and analysis of breast cancer cells. Attachment and spreading of MDA-MB-231 human metastatic breast cancer cells was compared to that of non-tumorigenic human breast epithelial cells, MCF-10A, and the impact of SAHA (Vorinostat), a histone deacetylase (HDAC) inhibitor, on cell behaviors was ascertained. Our results showed the cancer cells attached to flat silicon and PECVD nitride-coated silicon more efficiently than non-cancer cells, and preferential cancer cell attachment was enhanced by SAHA. Fluorescent immunohistochemistry (IHC) revealed that SAHA stimulated actin stress fiber formation and focal adhesion to the substrates; atomic force microscopy (AFM) showed SAHA increased the cancer cell stiffness. Collectively, SAHA-induced biomechanical changes altered the cell morphology and mode of attachment to flat silicon and to three-dimensional silicon microstructures. This is the first report of the use of AFM to characterize the biomechanical effects of a HDAC inhibitor in cancer cells.
- ASME Nanotechnology Council
Effects of an Experimental Drug, Suberoylanilide Hydroxamic Acid (SAHA), on Attachment, Spreading, and Stiffness of Human Breast Cancer Cells on Silicon Substrates
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Strobl, JS, Nikkhah, M, Rhoades, R, & Agah, M. "Effects of an Experimental Drug, Suberoylanilide Hydroxamic Acid (SAHA), on Attachment, Spreading, and Stiffness of Human Breast Cancer Cells on Silicon Substrates." Proceedings of the ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. Houston, Texas, USA. February 7–10, 2010. pp. 161-162. ASME. https://doi.org/10.1115/NEMB2010-13037
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