Enzyme-linked immunosorbent assays (ELISA), one of the most common immunoassays, is widely used for detection and quantification of chemical and biological molecules and is becoming more and more important in clinical diagnostics, food safety testing, and environmental monitoring. A major challenge in developing the CD-ELISA is to split the flow (e.g., bio-reagents) evenly on the micro-channels. The Coriolis force resultant from CD rotation can disturb the flow in the splitter region and thus cause the failure mode in delivering the solution from each reservoir in a pre-specified manner. In this study, we investigate on the effects of inlet pressure and Coriolis force on the splitting ratio under two splitter structures. The analysis is based on the incompressible Navier-Stokes equation and the simulation results agree well with our experimental work.

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