Effective detection of circulating tumor cells (CTCs) can provide important diagnostic and prognostic information of metastatic cancer. However, CTCs are extremely rare and estimated to be only in the range of one tumor cell in the background of 106–109 normal blood cells, hindering clinically significant detection.[1–2] The specific capturing and potential enrichment of CTCs using anti-epithelial cell adhesion molecule (anti-EpCAM)[3] and selectin, respectively, inspire a biofunctionalized surface that mimics biological complexity may detect and isolate target cells at a greater sensitivity and specificity. This concept is supported by the initial physiological interactions between CTCs and endothelium in the bloodstream, which include concurrent rolling and stationary binding steps. Towards this aim, we investigated the following: i) two proteins with distinct biofunctions (selectin to induce rolling and anti-EpCAM to statically capture target cells) can be co-immobilized; ii) a combined rolling and stationary binding can be induced by the mixture of the proteins; and iii) the biomimetic combination enhances overall capture efficiency of the surface. As a proof-of-concept study for the hypothesis of enhanced separation capacity and capture efficiency using protein mixtures, the surfaces are tested using in vitro cell lines (MCF-7 cells as a CTC model and HL-60 cells as a leukocyte model) under flow conditions. The effects of the combination of rolling (E-selectin) and stationary binding (anti-EpCAM) on capture efficiency are compared to a surface functionalized solely with anti-EpCAM.
Skip Nav Destination
ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology
February 7–10, 2010
Houston, Texas, USA
Conference Sponsors:
- ASME Nanotechnology Council
ISBN:
978-0-7918-4392-5
PROCEEDINGS PAPER
Enhanced Tumor Cell Separation by Surfaces Functionalized With Combinations of Bioadhesive Proteins Available to Purchase
Ja Hye Myung,
Ja Hye Myung
University of Illinois at Chicago, Chicago, IL
Search for other works by this author on:
Cari A. Launiere,
Cari A. Launiere
University of Illinois at Chicago, Chicago, IL
Search for other works by this author on:
Khyati A. Gajjar,
Khyati A. Gajjar
University of Illinois at Chicago, Chicago, IL
Search for other works by this author on:
David T. Eddington,
David T. Eddington
University of Illinois at Chicago, Chicago, IL
Search for other works by this author on:
Seungpyo Hong
Seungpyo Hong
University of Illinois at Chicago, Chicago, IL
Search for other works by this author on:
Ja Hye Myung
University of Illinois at Chicago, Chicago, IL
Cari A. Launiere
University of Illinois at Chicago, Chicago, IL
Khyati A. Gajjar
University of Illinois at Chicago, Chicago, IL
David T. Eddington
University of Illinois at Chicago, Chicago, IL
Seungpyo Hong
University of Illinois at Chicago, Chicago, IL
Paper No:
NEMB2010-13210, pp. 125-126; 2 pages
Published Online:
December 22, 2010
Citation
Myung, JH, Launiere, CA, Gajjar, KA, Eddington, DT, & Hong, S. "Enhanced Tumor Cell Separation by Surfaces Functionalized With Combinations of Bioadhesive Proteins." Proceedings of the ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. Houston, Texas, USA. February 7–10, 2010. pp. 125-126. ASME. https://doi.org/10.1115/NEMB2010-13210
Download citation file:
9
Views
Related Proceedings Papers
Related Articles
Pre-Clinical Evaluation of Direct Current Ablation for the Treatment of Benign Prostatic Hyperplasia
J. Med. Devices (June,2009)
Biotransport Special Issue
J Biomech Eng (July,2009)
Numerical Simulation of the Thermal Fields Occurring in the Treatment of Malignant Tumors by Local Hyperthermia
J Biomech Eng (August,1993)
Related Chapters
Introduction
Mechanical Blood Trauma in Circulatory-Assist Devices
Experimental Studies
Nanoparticles and Brain Tumor Treatment
Two Advanced Methods
Applications of Mathematical Heat Transfer and Fluid Flow Models in Engineering and Medicine