Layer-by-layer deposition of polyelectrolytes form multiple layers in the nanometer scale. Charged magnetic [1] and biomolecular [2] nano-colloids can be incorporated in to the layers. These multilayer assemblies are formed on the inner walls of a microchannel using laminar flow; alternatively polyanions and polycations are pumped through the channel. Water is also pumped through, between the deposition of each layer, in order to wash away the un-absorbed polyelectrolytes. The first charged layer is formed by silanation. The microfluidic device consists of a ‘T’ junction as shown in Fig. 1. Switching flow between polyelectrolytes and water is controlled by three syringe pumps, automated through a Labview virtual instrument.

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