Abstract
The emerging field of 3D bioprinting has ignited a demand for affordable and customizable 3D printers capable of utilizing bio-inks across various scales. This study presents a comprehensive framework for a specialized nozzle-holding device tailored for an extrusion-based 3D bioprinter, fine-tuned to meet the exacting demands of tissue engineering. The proposed system integrates a pneumatically driven plunger mechanism with an adaptive nozzle system, ensuring secure containment of bio-ink in a biologically safe container. Stringent measures are implemented to maintain a consistently low heat temperature, preserving the safety and integrity of the bio-ink. Central to this modification is a meticulously designed milled aluminum block, optimizing thermal characteristics while offering a protective barrier. An essential component is the 3D-printed extruder head bracket, produced with precision on a freeform resin printer, effectively mitigating potential heating-related discrepancies. The integration of these meticulously designed components culminates in a modified extrusion-based 3D bioprinter poised to revolutionize tissue engineering methodologies. The adaptive nozzle system ensures precise dispensing, and the choice of materials underscores a commitment to bio-ink integrity and thermal optimization. This study not only advances bioprinting technology but also highlights the pivotal role of innovative engineering in overcoming tissue engineering challenges. The proposed bioprinter design establishes a promising foundation for further research, aiming for more accurate, efficient, and secure bioprinting solutions.
