High-resolution 3D measurement is crucial for a wide range of applications in manufacturing. With the recent development of optical technologies, the performance of many 3D scanning systems has entered the practical range for object digitizing, reverse engineering, quality control, and many other manufacturing applications. In order to extend the measurement capability to reflective or transparent surfaces, a common practice for reducing the unwanted reflection and refraction is to coat the surfaces with micro-particle spray. There is, however, limited discussion about the influence of coating spray on the resulted measurement precision of 3D optical scanning systems, and due to lack of standardized procedure for spray coating, the variability caused by different operators in surface measurement remains to be examined. This paper investigates the 3D data acquisition of spray-coated surfaces with a structured-light scanning system through experiments and statistical analysis. Both surface roughness and spatial statistics are used to quantitatively evaluate the characteristics of the 3D measurement system. Gauge R&R study is conducted to determine measurement repeatability and reproducibility. The results demonstrate that both the number of spray pass and the skill of the individual operator can significantly affect the performance of the structured-light scanning system. Other pertinent suggestions for the operation of 3D optical scanning systems with spray coating are also provided.