Due to the need of high speed and efficient biodosimetric assays for triage and therapy in the event of radiological or nuclear attack, a robotically-based automated biodosimetry tool (RABiT) has been developed over the past few years. Adapting the micronucleus assay from filter plates to V-shaped plates presented challenges in the liquid handling, namely cell splashing out of the V-shaped well plate during the cell harvesting, poor cell distribution on the bottom of the image plate during the dispensing, and cell loss from the image plate during the aspiration in the liquid handling process. Experimental and numerical investigations were carried out to better understand the phenomena and mitigate the problems. Surface tension and contact angle among the fluids and the plate wall were accounted for in the discrete and multiphase numerical models. Experimental conditions were optimized based on the numerical results showing the relationship between nozzle speed and amount of splashed liquid, and the relationship between aspiration speed and number of escaped cells. Using these optimized parameters, numbers of micronuclei in binucleated cells showed the same dose dependence in the RABiT-prepared samples as those in the manually prepared ones. Micronucleus assay protocol was fully realized on RABiT.
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ASME 2016 11th International Manufacturing Science and Engineering Conference
June 27–July 1, 2016
Blacksburg, Virginia, USA
Conference Sponsors:
- Manufacturing Engineering Division
ISBN:
978-0-7918-4990-3
PROCEEDINGS PAPER
Platform-Dependent Liquid Handling in High-Throughput Biodosimetry Tool Available to Purchase
Jason C. Tsui,
Jason C. Tsui
Columbia University, New York, NY
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Mikhail Repin,
Mikhail Repin
Columbia University Medical Center, New York, NY
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Guy Garty,
Guy Garty
Columbia University Medical Center, New York, NY
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Helen Turner,
Helen Turner
Columbia University Medical Center, New York, NY
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Y. Lawrence Yao,
Y. Lawrence Yao
Columbia University, New York, NY
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David J. Brenner
David J. Brenner
Columbia University Medical Center, New York, NY
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Dakai Bian
Columbia University, New York, NY
Jason C. Tsui
Columbia University, New York, NY
Mikhail Repin
Columbia University Medical Center, New York, NY
Guy Garty
Columbia University Medical Center, New York, NY
Helen Turner
Columbia University Medical Center, New York, NY
Y. Lawrence Yao
Columbia University, New York, NY
David J. Brenner
Columbia University Medical Center, New York, NY
Paper No:
MSEC2016-8513, V002T03A007; 10 pages
Published Online:
September 27, 2016
Citation
Bian, D, Tsui, JC, Repin, M, Garty, G, Turner, H, Yao, YL, & Brenner, DJ. "Platform-Dependent Liquid Handling in High-Throughput Biodosimetry Tool." Proceedings of the ASME 2016 11th International Manufacturing Science and Engineering Conference. Volume 2: Materials; Biomanufacturing; Properties, Applications and Systems; Sustainable Manufacturing. Blacksburg, Virginia, USA. June 27–July 1, 2016. V002T03A007. ASME. https://doi.org/10.1115/MSEC2016-8513
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