The current study presents a new integrated microfluidic chip for rapid ribonucleic acid (RNA) purification, extraction and reverse transcription (RT) in an automatic fashion. The miniature system consists of two individual functional devices including a two-way microfluidic control module and a magnetic field/temperature control module. The functional microfluidic control module can perform pumping, mixing, purification and concentration of the RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bio-separators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT process. Experimental results showed that the total RNA was successfully purified and extracted by the magnetic beads, and the subsequent RT process of RNA was completed automatically. As a whole, the developed system may provide a powerful platform for biomedical application and biological analysis.
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ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer
June 6–9, 2008
Tainan, Taiwan
Conference Sponsors:
- Nanotechnology Institute
ISBN:
0-7918-4292-4
PROCEEDINGS PAPER
Bead-Based Miniature Microfluidic Systems for Rapid RNA Extraction and Reverse Transcription
Kang-Yi Lien,
Kang-Yi Lien
National Cheng Kung University, Tainan, Taiwan
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Chien-Ju Liu,
Chien-Ju Liu
National Cheng Kung University, Tainan, Taiwan
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Jyh-Wei Shin,
Jyh-Wei Shin
National Cheng Kung University, Tainan, Taiwan
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Tsuey-Yu Chang,
Tsuey-Yu Chang
National Cheng Kung University, Tainan, Taiwan
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Gwo-Bin Lee
Gwo-Bin Lee
National Cheng Kung University, Tainan, Taiwan
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Kang-Yi Lien
National Cheng Kung University, Tainan, Taiwan
Chien-Ju Liu
National Cheng Kung University, Tainan, Taiwan
Jyh-Wei Shin
National Cheng Kung University, Tainan, Taiwan
Tsuey-Yu Chang
National Cheng Kung University, Tainan, Taiwan
Gwo-Bin Lee
National Cheng Kung University, Tainan, Taiwan
Paper No:
MNHT2008-52268, pp. 251-256; 6 pages
Published Online:
June 22, 2009
Citation
Lien, K, Liu, C, Shin, J, Chang, T, & Lee, G. "Bead-Based Miniature Microfluidic Systems for Rapid RNA Extraction and Reverse Transcription." Proceedings of the ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer. ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer, Parts A and B. Tainan, Taiwan. June 6–9, 2008. pp. 251-256. ASME. https://doi.org/10.1115/MNHT2008-52268
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