We presented two kinds of innovative velocimetry with high temporal and spatial resolution respectively based on the Laser Induced Fluorescence Photobleaching Anemometer (LIFPA) and Stimulated Emission Depletion (STED) techniques. The temporal and spatial resolution has been for the first time achieved to 5–10 μs and 70 nm, respectively. To our knowledge, the temporal resolution is about 100× better than that of the state of the art microPIV, which is currently the most widely used velocimetry in microfluidics community. And for the first time, flow velocity distribution in a nanocapillary has been measured with a spatial resolution better than 70 nm.

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