For many bioanalytical systems, the quality of the sample under scrutiny greatly affects the success of its analysis by the analytical instrument. Thus, sample preparatory techniques such as solid-phase extraction (SPE), purification and concentration are used to improve the quality of the sample before introduction into the equipment. This work overviews our effort in developing microdevices which exploit thermally responsive aptamers for biomolecular sample extraction, purification and concentration. We demonstrate the feasibility of this approach with a model system which consists of an adenosine monophosphate (AMP) analyte and adenosine triphosphate derived aptamer. Through systematic experimentation, we demonstrate the extraction and enrichment of AMP at physiologically relevant concentrations, release of AMP and regeneration of the aptamer through thermal stimulation, and detection of AMP by either fluorescence or mass spectrometry. In addition, completely aqueous operation of the device eliminates the use of potentially harsh reagents.
Skip Nav Destination
Close
Sign In or Register for Account
2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems
June 3–5, 2008
Clear Water Bay, Kowloon, Hong Kong
Conference Sponsors:
- Nanotechnology Institute
ISBN:
0-7918-4294-0
PROCEEDINGS PAPER
Thermally Responsive Aptamer Surfaces for Microfluidic Sample Preparation
ThaiHuu Nguyen,
ThaiHuu Nguyen
Columbia University, New York, NY
Search for other works by this author on:
Qiao Lin
Qiao Lin
Columbia University, New York, NY
Search for other works by this author on:
ThaiHuu Nguyen
Columbia University, New York, NY
Qiao Lin
Columbia University, New York, NY
Paper No:
MicroNano2008-70264, pp. 209-215; 7 pages
Published Online:
June 12, 2009
Citation
Nguyen, T, & Lin, Q. "Thermally Responsive Aptamer Surfaces for Microfluidic Sample Preparation." Proceedings of the 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems. 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems. Clear Water Bay, Kowloon, Hong Kong. June 3–5, 2008. pp. 209-215. ASME. https://doi.org/10.1115/MicroNano2008-70264
Download citation file:
- Ris (Zotero)
- Reference Manager
- EasyBib
- Bookends
- Mendeley
- Papers
- EndNote
- RefWorks
- BibTex
- ProCite
- Medlars
Close
Sign In
3
Views
0
Citations
Related Proceedings Papers
Related Articles
Comparison of Experiments and Simulation of Joule Heating in ac Electrokinetic Chips
J. Fluids Eng (February,2010)
A Dedicated Low-Cost Fluorescence Microfluidic Device Reader for Point-of-Care Ocular Diagnostics
J. Med. Devices (June,2013)
Surface Temperature Measurement Using a Laser-Induced Fluorescence Thermal Imaging System
J. Heat Transfer (February,1994)
Related Chapters
Surface Analysis and Tools
Tribology of Mechanical Systems: A Guide to Present and Future Technologies
Application of Kelvin Force Microscopy Measurements and Secondary Ion Mass Spectroscopy to Evidence Hydrogen Embrittlement for the 2024 Aluminum Alloy
International Hydrogen Conference (IHC 2012): Hydrogen-Materials Interactions
Raman Spectroscopy of Lithium Hydride: Effects of Hydrogen Deficiency and Moisture Corrosion on Fluorescence
International Hydrogen Conference (IHC 2012): Hydrogen-Materials Interactions