Fluorescence Detection of a Minute Particle Using Resin-Based Optical Total Analysis Systems Having a High Aspect Ratio Light Waveguide Core

A precise light waveguide combined total analysis system (TAS)[1] is thought to be one of the most powerful functional elements needed to realize a “ubiquitous healthcare” system. In accordance with this concept, we have proposed a fundamental structure of detecting forward and side scattered light from small particles illuminating laser power through light waveguide formed in a thin resin layer. Based on this concept, we demonstrated its effectiveness of detecting both forward and side scattered light in in-plane direction[2] (Fig.1) by using a trial-manufactured TAS chip. Acquiring further various physiological properties, it is necessary to illuminate them with smaller cross section size of cores of light waveguide, and furthermore, to construct fluorescence detecting systems.