Abstract

Recently, an electrostatic technique has been described which enhances EC adhesion and retention upon physiologic shear stress [1–2]. The results of the in vitro evaluation indicated that the optimum electrostatic seeding using human umbilical vein endothelial cells (HUVEC) was obtained at +1.0 Volt applied for a 16 minute seeding time. The optimum conditions resulted in an average EC seeding density of 73,540 HUVEC/cm2 with an average viable surface coverage of 97.4%. The average morphological maturation ranking represented all flattened HUVECs with an average damage ranking corresponding to minimal cellular membrane damage. The HUVEC retention study (in vitro) showed that the optimally electrostatic seeded grafts experienced no significant (α = 0.05) loss of HUVECs upon physiologic shear stress exposure (15 dynes/cm2). The biochemical assay results showed no significant (α = 0.05) production of prostacyclin or thromboxane A2 by either the gravitationally or electrostatically seeded HUVECs [2].

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