Arg-Gly-Asp-Ser (RGDS) coating is one of effective methods to improve the cell adhesive property of the scaffold surface. However, it is difficult to regulate the RGDS quantity and distribution, and to visualize RGDS distribution. The purpose of this study was to develop a surface treatment technique that the RGDS quantity can be regulated with the ultraviolet rays irradiation and the RGDS distribution can be visualized with the fluorescence. P(MMA-g-ANP-RGDS) and P(HEMA-g-ANP-RGDS) were respectively synthesized by radical copolymerization of methylmethacrylate (MMA) and 2-hydroxyethylmethacrylate (HEMA) with peptide-macromonomer containing photo-labile linker (3-amino-3-(2-nitrophenyl)propionic acid (ANP)) and RGDS. Each polymer film was produced by using spin-coater, and then ultraviolet rays was irradiated to the each film through the glass mask with three different ultraviolet rays transmissivity of 0 %, 30 % and 60 %. In both polymer films, the RGDS quantity can be regulated by ultraviolet rays irradiation, and the luminance decreased same as the RGDS quantity. Adherent osteoblast-like cells were not observed on P(HEMA-g-ANP-RGDS) film, but the number of adherent osteoblast-like cells was increased with increasing the RGDS quantity on the P(MMA-g-ANP-RGDS) film. In conclusion, we accomplished to develop the surface treatment technique with P(MMA-g-ANP-RGDS) to regulate and visualize the RGDS quantity and distribution.

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