The paper focuses on the effect of decalcification on microstructure and the mechanical and electrical properties of cortical bone. Decalcification is produced by placing the specimens into 5% vinegar acid for 72 hours. This acid treatment leads to a decrease in mass of the specimens 7.78 % (averaged over ten acid treated specimens). Microstructure of natural bone and acid treated bone is then compared using confocal microscopy. To estimate effect of acid treatment on electrical resistivity of bone, the specimens are rinsed and saturated with 0.9% NaCl solution for ten minutes. Then electrical resistance is measured by the four-point method and electrical resistivity is calculated. Averaging over ten acid treated specimens and ten control specimens show that decalcification lead to increase of electrical resistivity 5.85 times. Comparison of mechanical properties of natural and acid treated bones is done by three point bending using Instron 5882 testing machine. It is observed that 7.78 % mass loss in cortical bone yields reduction of the Young’s modulus about 2.7 times and bending strength of the specimens by 35%. A positive correlation between change in strength and Young’s modulus and electrical resistivity of the individual specimens is observed. The obtained results allows one to estimate changes in mechanical and electrical properties of bone from known losses in bone mass and, thus, non-destructively evaluate the decrease in bone strength through changes in electrical resistivity.

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