The human vocal folds are subjected to complex dynamic biomechanical stimulation during phonation. The aim of the present study was to develop and evaluate an airflow-induced self-oscillating mechanical model, i.e., a bioreactor, which mimics the geometry and the mechanical microenvironment of the human vocal folds. The bioreactor consisted of two composite synthetic vocal fold replicas loaded into a custom-built airflow supplied tube. A cell-scaffold mixture was injected into cavities within the replicas. The folds were phonated using a variable speed centrifugal blower for two hours a day over a period of seven days. The static and dynamic subglottal pressures and the dynamic supraglottal pressure were monitored. A similar bioreactor without mechanical excitation was used as positive control. The cell-scaffold mixture was harvested for cell viability and collagen type I immunohistochemistry tests seven days after injection. The flow-induced self-oscillations of the vocal fold replicas were shown to produce mechanical excitations that are typical of those in the human vocal fold lamina propria during phonation. The results confirmed that human vocal fold fibroblasts survived inside the present bioreactor, and maintained cellular functions of protein production.
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ASME 2014 International Mechanical Engineering Congress and Exposition
November 14–20, 2014
Montreal, Quebec, Canada
Conference Sponsors:
- ASME
ISBN:
978-0-7918-4646-9
PROCEEDINGS PAPER
Development of a Self-Oscillating Mechanical Model to Investigate the Biological Response of Human Vocal Fold Fibroblasts to Phono-Mimetic Stimulation
Neda Latifi,
Neda Latifi
McGill University, Montreal, QC, Canada
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Hossein K. Heris,
Hossein K. Heris
McGill University, Montreal, QC, Canada
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Siavash Kazemirad,
Siavash Kazemirad
McGill University, Montreal, QC, Canada
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Luc Mongeau
Luc Mongeau
McGill University, Montreal, QC, Canada
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Neda Latifi
McGill University, Montreal, QC, Canada
Hossein K. Heris
McGill University, Montreal, QC, Canada
Siavash Kazemirad
McGill University, Montreal, QC, Canada
Luc Mongeau
McGill University, Montreal, QC, Canada
Paper No:
IMECE2014-38970, V003T03A002; 7 pages
Published Online:
March 13, 2015
Citation
Latifi, N, Heris, HK, Kazemirad, S, & Mongeau, L. "Development of a Self-Oscillating Mechanical Model to Investigate the Biological Response of Human Vocal Fold Fibroblasts to Phono-Mimetic Stimulation." Proceedings of the ASME 2014 International Mechanical Engineering Congress and Exposition. Volume 3: Biomedical and Biotechnology Engineering. Montreal, Quebec, Canada. November 14–20, 2014. V003T03A002. ASME. https://doi.org/10.1115/IMECE2014-38970
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