In light of the significance of the viscoelastic property of agar to cell-based tissue engineering, this paper presents the stress relaxation measurement of agar using a polymer-based microfluidic device. Comprised of a single polymer rectangular microstructure and a set of electrolyte-enabled distributed transducers, this device is capable of detecting continuous distributed static and dynamic loads. In the measurement, an agar specimen is placed on the device and a rigid probe is utilized to press the specimen against the device with a step displacement input. Consequently, the stress relaxation behavior of the specimen translates to time-dependent continuous distributed loads acting on the device and is further registered as discrete resistance changes by the device. Two agar specimens of 1% and 3% in concentration, respectively, are measured using this device; and the data analysis is conducted on the measured results to extract Young’s relaxation modulus, which is further expressed by a Prony-series representation of the Maxwell model with two exponential terms. The results demonstrate the feasibility of using this device to measure the stress relaxation behavior of soft materials.

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