This paper reports programmable micropatterning of electrospun nanofibrous materials using a collector chip that consists of an array of independently controllable microelectrodes. The microelectrodes on the collecting chip are prepared by standard photolithography. By programming the local electrical field using excited and floating electrodes, the collector chip allows patterning of microstructures with controllable characteristics. The difference of electrostatic force between the excited and the floating electrodes increases the patterning contrast of electrospun nanofibers. The arbitrary geometries are successfully patterned on the array of 6 × 6 electrodes by independently programmable control of the voltage of each electrode. The experimental result also shows that it is possible to control the porosity and alignment of fibers. This method provides a simple yet highly reliable approach for creating combined micro/nanostructures of polymer nanofibers in a cost effective manner, which has great potential in functional tissue engineering, filtration, and chemical sensing. The work is also expected to foster the use of nanofibers in microdevices for on-chip biochemical analysis, and controlled infiltration and proliferation. The resulting nanofibers with controllable porosity are especially useful for the construction of tissue engineering scaffolds with morphological and functional similarity with natural tissues.

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