We present a novel immobilization technique via physical adsorption of biomolecules onto a sensing surface. The immobilization technique utilizes the competitive nature of protein adsorption onto the surface, offering the selectivity and sensitivity in detecting a target molecule. The SPR (Surface Plasmon Resonance) sensorgrams show that three human serum proteins, albumin, Immunoglobulin G (IgG) and fibrinogen, have different adsorption strengths to the surface and the competitive adsorption of individuals controls the exchange sequence. Using the competitive adsorption and exchange, we demonstrate that IgG is selectively and sensitively detected.
A Simple Interfacial Design of Biomolecules to Nano/Micro-Devices for Highly Sensitive and Selective Protein Detection
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Choi, S, & Chae, J. "A Simple Interfacial Design of Biomolecules to Nano/Micro-Devices for Highly Sensitive and Selective Protein Detection." Proceedings of the ASME 2009 International Mechanical Engineering Congress and Exposition. Volume 12: Micro and Nano Systems, Parts A and B. Lake Buena Vista, Florida, USA. November 13–19, 2009. pp. 811-814. ASME. https://doi.org/10.1115/IMECE2009-12575
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