Flow-Through Nanohole Array Based Sensing

Integration of nanohole array based sensing in microfluidic environments has been focus of several recent works. However, nanohole array based sensing performed to date has involved dead-ended holes that fail to harness the potential benefits provided by flow-through operation. Potential benefits include enhanced transport of reactants via nanoconfinement and solution sieving. Here, we describe computer based analysis related with nanofluidic transport and solid mechanics under a flow-through sensing scheme. We also demonstrate experimental nanofluidic flow-through transport and its application to real-time monitoring of self-assembled monolayer creation and biomarker detection.