We propose a hydrodynamic focusing based particle filtration method. Three microchannels associated for the filtration were networked with an island structure to control a boundary to limit particle sizes. The boundary was easily controlled by adjusting lengths of the three microchannels. Using hydrodynamic focusing in the microfluidic network, we have successfully designed and tested the device to filtrate particles of 10-μm and 20-μm. By adjusting the ratios of hydraulic resistances (or outlet channel lengths) using the built-in valves, the boundary distance was controlled. The proposed method could continuously sort particles by size. Thus, the proposed sorting method can be applicable for many fields for biology and biomedical engineering.

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